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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [1]
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- Product number
- PA1004 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Alkaline Phosphatase/ALPL Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Alkaline Phosphatase/ALPL detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Alkaline Phosphatase/ALPL information: Molecular Weight: 57305 MW; Subcellular Localization: Cell membrane ; Lipid-anchor, GPI-anchor .
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Network pharmacological study and in vitro studies validation-Molecular dynamics simulation of Cistanche deserticola in promoting periodontitis and bone remodeling.
WISP2 downregulation inhibits the osteogenic differentiation of BMSCs in congenital scoliosis by regulating Wnt/β-catenin pathway.
Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats.
Electrospinning Nanofiber-Reinforced Aerogels for the Treatment of Bone Defects.
Less Vertebral Bone Mass after Treatment with Macitentan in Mice: A Pilot Study.
Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels.
Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro.
MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR.
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor.
Preparation, physicochemical properties and biocompatibility of PBLG/PLGA/bioglass composite scaffolds.
Decreasing the ratio of matriptase/HAI‑1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer.
A comparison of osteocyte bioactivity in fine particulate bone powder grafts vs larger bone grafts in a rat bone repair model.
Prevention of osteoporosis in mice after ovariectomy via allograft of microencapsulated ovarian cells.
Shan C, Wu Z, Xia Y, Ji X, Zhang W, Peng X, Zhao J
International immunopharmacology 2024 Jun 30;135:112299
International immunopharmacology 2024 Jun 30;135:112299
WISP2 downregulation inhibits the osteogenic differentiation of BMSCs in congenital scoliosis by regulating Wnt/β-catenin pathway.
Zheng Y, Shen P, Tong M, Li H, Ren C, Wu F, Li H, Yang H, Cai B, Du W, Zhao X, Yao S, Quan R
Biochimica et biophysica acta. Molecular basis of disease 2023 Oct;1869(7):166783
Biochimica et biophysica acta. Molecular basis of disease 2023 Oct;1869(7):166783
Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats.
Hou C, Wang X, Jiang W, Bian Z, Zhu L, Li M
International journal of molecular medicine 2021 Aug;48(2)
International journal of molecular medicine 2021 Aug;48(2)
Electrospinning Nanofiber-Reinforced Aerogels for the Treatment of Bone Defects.
Zhang Y, Yin C, Cheng Y, Huang X, Liu K, Cheng G, Li Z
Advances in wound care 2020 Aug;9(8):441-452
Advances in wound care 2020 Aug;9(8):441-452
Less Vertebral Bone Mass after Treatment with Macitentan in Mice: A Pilot Study.
Liu ZY, Au MT, He TW, Yang B, Liu B, Zhang LM, Luo CX, Rong LM, Wen CY
BioMed research international 2019;2019:2075968
BioMed research international 2019;2019:2075968
Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels.
Jiang T, Zhao J, Yu S, Mao Z, Gao C, Zhu Y, Mao C, Zheng L
Biomaterials 2019 Jan;188:130-143
Biomaterials 2019 Jan;188:130-143
Bone morphogenetic protein 7 promotes odontogenic differentiation of dental pulp stem cells in vitro.
Zhu L, Ma J, Mu R, Zhu R, Chen F, Wei X, Shi X, Zang S, Jin L
Life sciences 2018 Jun 1;202:175-181
Life sciences 2018 Jun 1;202:175-181
MiR-351 negatively regulates osteoblast differentiation of MSCs induced by (+)-cholesten-3-one through targeting VDR.
Hou Q, Huang Y, Luo Y, Wang B, Liu Y, Deng R, Zhang S, Liu F, Chen D
American journal of translational research 2017;9(11):4963-4973
American journal of translational research 2017;9(11):4963-4973
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor.
Hou QK, Huang YQ, Luo YW, Wang B, Liu YM, Deng RD, Zhang SX, Lai YT, Li WY, Chen DF
Experimental and therapeutic medicine 2017 May;13(5):1841-1849
Experimental and therapeutic medicine 2017 May;13(5):1841-1849
Preparation, physicochemical properties and biocompatibility of PBLG/PLGA/bioglass composite scaffolds.
Cui N, Qian J, Wang J, Ji C, Xu W, Wang H
Materials science & engineering. C, Materials for biological applications 2017 Feb 1;71:118-124
Materials science & engineering. C, Materials for biological applications 2017 Feb 1;71:118-124
Decreasing the ratio of matriptase/HAI‑1 by downregulation of matriptase as a potential adjuvant therapy in ovarian cancer.
Sun P, Jiang Z, Chen X, Xue L, Mao X, Ruan G, Song Y, Mustea A
Molecular medicine reports 2016 Aug;14(2):1465-74
Molecular medicine reports 2016 Aug;14(2):1465-74
A comparison of osteocyte bioactivity in fine particulate bone powder grafts vs larger bone grafts in a rat bone repair model.
Sun YX, Sun CL, Tian Y, Xu WX, Zhou CL, Xi CY, Yan JL, Wang XT
Acta histochemica 2014 Jul;116(6):1015-21
Acta histochemica 2014 Jul;116(6):1015-21
Prevention of osteoporosis in mice after ovariectomy via allograft of microencapsulated ovarian cells.
Guo XX, Zhou JL, Xu Q, Lu X, Liang YJ, Weng J, Shi XL
Anatomical record (Hoboken, N.J. : 2007) 2010 Feb;293(2):200-7
Anatomical record (Hoboken, N.J. : 2007) 2010 Feb;293(2):200-7
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Anti-Alkaline Phosphatase antibody, PA1004, Western blottingAll lanes: Anti ALPL (PA1004) at 0.5ug/mlWB: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 57KDObserved bind size: 57KD
- Additional image
