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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C375254 - Provider product page
- Provider
- LSBio
- Product name
- ARG2 / Arginase 2 Antibody (aa1-354) LS-C375254
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot. All lanes: Arginase-2, mitochondrial antibody at 5 ug/ml. Lane 1: mouse liver tissue. Lane 2: 293T whole cell lysate. Lane 3: mouse kidney tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 39 kDa. Observed band size: 39 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating ARG2 in HEK293 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of ARG2 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ARG2 Antibody (8µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (10µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating ARG2 in HEK293 whole cell lysate Lane 1: Rabbit control IgG (1µg) instead of ARG2 Antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: ARG2 Antibody (8µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (10µg)
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate using antibody at 1:100 dilution.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using antibody at 1:100 dilution.
