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LearnA300-649A
antibody from Invitrogen Antibodies
Targeting: BHLHE40
BHLHB2, Clast5, DEC1, SHARP2, STRA13
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A300-649A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DEC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DEC1 by western blot. Samples: Whole cell lysate (50 µg) from HDLM-2, GaMG, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DEC1 antibody (Product # A300-649A lot 3) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DEC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DEC1 antibody A300-649A (lot A300-649A-2) used for IP at 6 µg per reaction. DEC1 was also immunoprecipitated by rabbit anti-DEC1 antibody BL2927. For blotting immunoprecipitated DEC1, A300-649A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DEC1 by western blot. Samples: Whole cell lysate (50, 15 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DEC1 antibody A300-649A (lot A300-649A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DEC1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DEC1 (Product # A300-649A; lot 3) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DEC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DEC1 (Cat. No. A300-649A Lot2) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DEC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DEC1 antibody (Product # A300-649A lot 3) used for IP at 6 µg per reaction. DEC1 was also immunoprecipitated by a second antibody against a different epitope of DEC1 (BL2927). For blotting immunoprecipitated DEC1 (Product # A300-649A) was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
