- NB100-1800 - Novus Biologicals BHLHE40 antibody

Submitted references Role of Systemic Lupus Erythematosus Risk Variants With Opposing Functional Effects as a Driver of Hypomorphic Expression of TNIP1 and Other Genes Within a Three-Dimensional Chromatin Network.

Pasula S, Tessneer KL, Fu Y, Gopalakrishnan J, Pelikan RC, Kelly JA, Wiley GB, Wiley MM, Gaffney PM
Arthritis & rheumatology (Hoboken, N.J.) 2020 May;72(5):780-790

The Transcription Factor Bhlhe40 Programs Mitochondrial Regulation of Resident CD8⁺ T Cell Fitness and Functionality.

Li C, Zhu B, Son YM, Wang Z, Jiang L, Xiang M, Ye Z, Beckermann KE, Wu Y, Jenkins JW, Siska PJ, Vincent BG, Prakash YS, Peikert T, Edelson BT, Taneja R, Kaplan MH, Rathmell JC, Dong H, Hitosugi T, Sun J
Immunity 2019 Sep 17;51(3):491-507.e7

Dec1 Deficiency Suppresses Cardiac Perivascular Fibrosis Induced by Transverse Aortic Constriction.

Le HT, Sato F, Kohsaka A, Bhawal UK, Nakao T, Muragaki Y, Nakata M
International journal of molecular sciences 2019 Oct 8;20(19)

Bhlhe40 mediates tissue-specific control of macrophage proliferation in homeostasis and type 2 immunity.

Jarjour NN, Schwarzkopf EA, Bradstreet TR, Shchukina I, Lin CC, Huang SC, Lai CW, Cook ME, Taneja R, Stappenbeck TS, Randolph GJ, Artyomov MN, Urban JF Jr, Edelson BT
Nature immunology 2019 Jun;20(6):687-700

Smad3 Suppresses Epithelial Cell Migration and Proliferation via the Clock Gene Dec1, Which Negatively Regulates the Expression of Clock Genes Dec2 and Per1.

Sato F, Otsuka T, Kohsaka A, Le HT, Bhawal UK, Muragaki Y
The American journal of pathology 2019 Apr;189(4):773-783

Mice lacking the transcriptional regulator Bhlhe40 have enhanced neuronal excitability and impaired synaptic plasticity in the hippocampus.

Hamilton KA, Wang Y, Raefsky SM, Berkowitz S, Spangler R, Suire CN, Camandola S, Lipsky RH, Mattson MP
PloS one 2018;13(5):e0196223

Differentiated embryo chondrocyte plays a crucial role in DNA damage response via transcriptional regulation under hypoxic conditions.

Nakamura H, Bono H, Hiyama K, Kawamoto T, Kato Y, Nakanishi T, Nishiyama M, Hiyama E, Hirohashi N, Sueoka E, Poellinger L, Tanimoto K
PloS one 2018;13(2):e0192136

Bhlhe40 is an essential repressor of IL-10 during Mycobacterium tuberculosis infection.

Huynh JP, Lin CC, Kimmey JM, Jarjour NN, Schwarzkopf EA, Bradstreet TR, Shchukina I, Shpynov O, Weaver CT, Taneja R, Artyomov MN, Edelson BT, Stallings CL
The Journal of experimental medicine 2018 Jul 2;215(7):1823-1838

DEC1 negatively regulates the expression of CXCL10 and CCL5 induced by poly IC in normal human mesangial cells.

Liu Q, Imaizumi T, Murakami K, Tanaka H, Wu Y, Yoshizawa T, Morohashi S, Seino H, Kijima H
Biomedical research (Tokyo, Japan) 2017;38(4):249-255

DEC1 promotes hypoxia-induced epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma cells.

Murakami K, Wu Y, Imaizumi T, Aoki Y, Liu Q, Yan X, Seino H, Yoshizawa T, Morohashi S, Kato Y, Kijima H
Biomedical research (Tokyo, Japan) 2017;38(4):221-227

DEC2 expression antagonizes cisplatin‑induced apoptosis in human esophageal squamous cell carcinoma.

Sato H, Wu Y, Kato Y, Liu Q, Hirai H, Yoshizawa T, Morohashi S, Watanabe J, Kijima H
Molecular medicine reports 2017 Jul;16(1):43-48

Smad3 and Bmal1 regulate p21 and S100A4 expression in myocardial stromal fibroblasts via TNF-α.

Sato F, Kohsaka A, Takahashi K, Otao S, Kitada Y, Iwasaki Y, Muragaki Y
Histochemistry and cell biology 2017 Dec;148(6):617-624

Rhythmic expression of DEC2 protein in vitro and in vivo.

Sato F, Muragaki Y, Kawamoto T, Fujimoto K, Kato Y, Zhang Y
Biomedical reports 2016 Jun;4(6):704-710

IL-1-induced Bhlhe40 identifies pathogenic T helper cells in a model of autoimmune neuroinflammation.

Lin CC, Bradstreet TR, Schwarzkopf EA, Jarjour NN, Chou C, Archambault AS, Sim J, Zinselmeyer BH, Carrero JA, Wu GF, Taneja R, Artyomov MN, Russell JH, Edelson BT
The Journal of experimental medicine 2016 Feb 8;213(2):251-71

USP17- and SCFβTrCP--regulated degradation of DEC1 controls the DNA damage response.

Kim J, D'Annibale S, Magliozzi R, Low TY, Jansen P, Shaltiel IA, Mohammed S, Heck AJ, Medema RH, Guardavaccaro D
Molecular and cellular biology 2014 Nov 15;34(22):4177-85

Wnt signaling potentiates nevogenesis.

Pawlikowski JS, McBryan T, van Tuyn J, Drotar ME, Hewitt RN, Maier AB, King A, Blyth K, Wu H, Adams PD
Proceedings of the National Academy of Sciences of the United States of America 2013 Oct 1;110(40):16009-14

The transcription factor DEC1 (BHLHE40/STRA13/SHARP-2) is negatively associated with TNM stage in non-small-cell lung cancer and inhibits the proliferation through cyclin D1 in A549 and BE1 cells.

Liu Y, Wang L, Lin XY, Wang J, Yu JH, Miao Y, Wang EH
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2013 Jun;34(3):1641-50

Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma.

Ma W, Shi X, Lu S, Wu L, Wang Y
Oncology reports 2013 Dec;30(6):2957-62

Differential hypoxic regulation of hypoxia-inducible factors 1alpha and 2alpha.

Lin Q, Cong X, Yun Z
Molecular cancer research : MCR 2011 Jun;9(6):757-65

Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells.

Liu Y, Sato F, Kawamoto T, Fujimoto K, Morohashi S, Akasaka H, Kondo J, Wu Y, Noshiro M, Kato Y, Kijima H
Genes to cells : devoted to molecular & cellular mechanisms 2010 Apr 1;15(4):315-25

Identification of potential stroke targets by lentiviral vector mediated overexpression of HIF-1 alpha and HIF-2 alpha in a primary neuronal model of hypoxia.

Ralph GS, Parham S, Lee SR, Beard GL, Craigon MH, Ward N, White JR, Barber RD, Rayner W, Kingsman SM, Mundy CR, Mazarakis ND, Krige D
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2004 Feb;24(2):245-58

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DEC1 Antibody [NB100-1800] - Western blot analysis of DEC1 in HeLa whole cell lysate.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: DEC1 Antibody [NB100-1800] - Simple Western lane view shows a specific band for DEC1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: DEC1 Antibody [NB100-1800] - Western Blot Image of anti-DEC1. Whole cell protein from A431 (lane 1) and PC3 (lane 2) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-DEC1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoprecipitation: DEC1 Antibody [NB100-1800] - Samples: Whole cell lysate from HeLa (50 ug for WB) and 293T (1 mg/IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-DEC1 antibody BL2928 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and for IP at 3 ug/mg lysate. DEC1 was also immunoprecipitated using rabbit anti-DEC1 antibodies BL2926 and BL2927, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: DEC1 Antibody [NB100-1800] - P. gingivalis-challenged rat maxilla using DEC1 antibody at 1:100. Heat mediated antigen retrieval (sodium citrate buffer pH 6.0) was performed. Counter-stained with hematoxylin. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: DEC1 Antibody [NB100-1800] - An intracellular stain was performed on A431 cells with DEC1 Antibody NB100-1800AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoblotting: DEC1 Antibody [NB100-1800] - DNA-DRR genes promoter activities decreased with DEC. (B) The DEC1 or DEC2 expression plasmids (B), each reporter, and DEC1 or delta basic DEC1 expression plasmids (C) were co-transfected into HepG2. Under normoxic conditions they were then incubated for 36 hours. A dual-luciferase assay system determined luciferase activities. Citation: Nakamura H, Bono H, Hiyama K, Kawamoto T, Kato Y, Nakanishi T, et al. (2018) Differentiated embryo chondrocyte plays a crucial role in DNA damage response via transcriptional regulation under hypoxic conditions. PLoS ONE 13(2): e0192136. https://doi.org/10.1371/journal.pone.0192136

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoblotting: DEC1 Antibody [NB100-1800] - (B) HSC-2 cells were used to perform knockdown assays for three primary antibodies: HIF1A, DEC1, and DEC2 9. Real-time RT-PCR evaluated expression levels of MSH2, MBD4, MRE11A, BRCA1, and RAD51. Value displayed above the bar represents the ratio of expression under hypoxic conditions compared with that under normoxia. Citation: Nakamura H, Bono H, Hiyama K, Kawamoto T, Kato Y, Nakanishi T, et al. (2018) Differentiated embryo chondrocyte plays a crucial role in DNA damage response via transcriptional regulation under hypoxic conditions. PLoS ONE 13(2): e0192136. https://doi.org/10.1371/journal.pone.0192136

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunoblotting: DEC1 Antibody [NB100-1800] - Knockdown assays in HSC-2 cells for HIF1A, DEC1, and DEC2. After transfecting and culturing non-specific siRNA or targeted siRNA into HSC-2, culturing them under normoxic and hypoxic conditions (24 hours), RT-PCR evaluated expression levels of the three primary antibodies. Citation: Nakamura H, Bono H, Hiyama K, Kawamoto T, Kato Y, Nakanishi T, et al. (2018) Differentiated embryo chondrocyte plays a crucial role in DNA damage response via transcriptional regulation under hypoxic conditions. PLoS ONE 13(2): e0192136. https://doi.org/10.1371/journal.pone.0192136

NB100-1800

Antibody data