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LearnPA1-16546
antibody from Invitrogen Antibodies
Targeting: BHLHE40
BHLHB2, Clast5, DEC1, SHARP2, STRA13
Western blot
ELISA
Immunoprecipitation Immunohistochemistry Flow cytometry Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA1-16546 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BHLHE40 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Suggested positive control: antigen standard for BHLHB2 (transient overexpression lysate).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of Human DEC1 on HeLa whole cell lystate using Product # PA1-16546. DEC1 was also immunoprecipitated using 2 other rabbit anti-DEC1 antibodies.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BHLHE40 in whole cell protein from A431 and PC3. Samples were incubated in BHLHE40 polyclonal antibody (Product # PA1-16546) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. A431 (lane 1) and PC3 (lane 2) was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BHLHE40 in HeLa whole cell lysate. Sample was incubated in BHLHE40 polyclonal antibody (Product # PA1-16546).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BHLHE40 in 1.0 mg/mL HeLa lysate. Samples were incubated in BHLHE40 polyclonal antibody (Product # PA1-16546). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of BHLHE40 in A431 cells. Samples were incubated in BHLHE40 polyclonal antibody (Product # PA1-16546) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of BHLHE40 was performed using HeLa and 293T cells. Antigen-antibody complexes were formed by incubating whole cell lysate from HeLa and 293T cells (50 µg for WB and 1 mg/IP, 20% of IP loaded for 293T cells) with BHLHE40 polyclonal antibody (Product # PA1-16546). Anti-DEC1 antibody used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
