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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX110275 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110275, RRID:AB_1951026
- Product name
- NSF antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX110275 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE GTX110275 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NSF antibody detects NSF protein by Western blot analysis.A. 50 µg rat brain lysate/extract7.5 % SDS-PAGENSF antibody (GTX110275) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody (GTX110275) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody (GTX110275) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NSF antibody detects NSF protein by immunofluorescent analysis.Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: NSF protein stained by NSF antibody (GTX110275) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NSF antibody detects NSF protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. NSF antibody (GTX110275) diluted at 1:500.
