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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-78095 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NSF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, mouse brain, rat brain
- Concentration
- 0.27 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NSF in mouse tissue extract using 50 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with NSF polyclonal antibody (Product # PA5-78095) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NSF in 293T cells using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with NSF polyclonal antibody (Product # PA5-78095) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NSF in rat tissue extract using 50 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with NSF polyclonal antibody (Product # PA5-78095) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NSF Polyclonal Antibody (Product # PA5-78095). Sample (30 µg of whole cell lysate). Lane A: 293T . 7.5% SDS PAGE. NSF Polyclonal Antibody (Product # PA5-78095) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NSF Polyclonal Antibody (Product # PA5-78095). Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF Polyclonal Antibody (Product # PA5-78095) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NSF Polyclonal Antibody (Product # PA5-78095). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF Polyclonal Antibody (Product # PA5-78095) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NSF Polyclonal Antibody detects NSF protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: NSF protein stained by NSF Polyclonal Antibody (Product # PA5-78095) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta-3 Tubulin Polyclonal Antibody [GT1338]diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NSF Polyclonal Antibody detects NSF protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. NSF Polyclonal Antibody (Product # PA5-78095) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
