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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3464 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3464, RRID:AB_1579345
- Product name
- PTK7 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PTK7.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.
Characterization of the human full-length PTK7 cDNA encoding a receptor protein tyrosine kinase-like molecule closely related to chick KLG.
Colon carcinoma kinase-4 defines a new subclass of the receptor tyrosine kinase family.
Zhang H, Li XJ, Martin DB, Aebersold R
Nature biotechnology 2003 Jun;21(6):660-6
Nature biotechnology 2003 Jun;21(6):660-6
Characterization of the human full-length PTK7 cDNA encoding a receptor protein tyrosine kinase-like molecule closely related to chick KLG.
Park SK, Lee HS, Lee ST
Journal of biochemistry 1996 Feb;119(2):235-9
Journal of biochemistry 1996 Feb;119(2):235-9
Colon carcinoma kinase-4 defines a new subclass of the receptor tyrosine kinase family.
Mossie K, Jallal B, Alves F, Sures I, Plowman GD, Ullrich A
Oncogene 1995 Nov 16;11(10):2179-84
Oncogene 1995 Nov 16;11(10):2179-84
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CCK4 (arrow) using rabbit PTK7 polyclonal antibody (Cat # PAB3464). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTK7 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PTK7 polyclonal antibody (Cat # PAB3464) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NCI-H460 cells using PTK7 polyclonal antibody (Cat # PAB3464)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
