TA503920

antibody from OriGene

Targeting: PTK7 CCK4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA503920

Provider

OriGene

Proper citation

OriGene Cat#TA503920, RRID:AB_11126353

Product name

PTK7 (CCK4) mouse monoclonal antibody, clone OTI2E7 (formerly 2E7)

Antibody type

Monoclonal

Description

PTK7 (CCK4) mouse monoclonal antibody, clone OTI2E7 (formerly 2E7)

Host

Mouse

Conjugate

Unconjugated

Epitope

PTK7

Isotype

IgG

Antibody clone number

OTI2E7

Vial size

100 µl

Concentration

NULL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PTK7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTK7 (RC223416, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTK7.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-PTK7 mouse monoclonal antibody (TA503920) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PTK7(RC223416).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC223416 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PTK7 antibody(TA503920), and then analyzed by flow cytometry.

Validation comment

FC