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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-97262 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTK7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: FLAKAQGLEE GVAETLVLVK SLQSKDEQQQ LDFRRELEMF GKLNHANVVR LLGLCREAEP HYMVLEYVDL GDLKQFLRIS KSKDEKLKSQ PLSTKQKVAL CTQVALGMEH LSNNRFVHKD LAARNCLVSA QRQVKVSALG LSKDVYNSEY YHFRQAWVPL RWMSPEAILE GDFSTKSDVW AFGVLMWEVF THGEMPHGGQ ADDEVLADLQ AGKARLPQPE GCPSKLYRLM QRCWALSPKD RPSFSEIASA LGDSTVDSKP; Positive Samples: NCI-H460, HepG2, SKOV3, Jurkat, Mouse lung, Mouse liver, Rat testis; Cellular Location: Cell junction, Membrane, Single-pass type I membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.28 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references In Vivo Evaluation of Sgc8-c Aptamer as a Molecular Imaging Probe for Colon Cancer in a Mouse Xenograft Model.
Arévalo AP, Castelli R, Ibarra M, Crispo M, Calzada V
International journal of molecular sciences 2022 Feb 23;23(5)
International journal of molecular sciences 2022 Feb 23;23(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PTK7 in extracts of various cell lines using PTK7 Polyclonal Antibody (Product # PA5-97262) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PTK7 in paraffin-embedded mouse brain using PTK7 Polyclonal Antibody (Product # PA5-97262) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PTK7 in paraffin-embedded human prostate using PTK7 Polyclonal Antibody (Product # PA5-97262) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PTK7 in paraffin-embedded human stomach using PTK7 Polyclonal Antibody (Product # PA5-97262) at a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Micrographs of hematoxylin and eosin (H&E), HER2, and PTK7 IHC staining of harvested LS174T tumor tissue. LS174T tumors were PTK7-positive and HER2-negative. ( A - C ) Tumor #1 showed minimal incomplete membrane expression for HER2 (score 1+) and diffuse cytoplasmic positivity for PTK7. ( D - F ) For tumor #2, HER2 expression was not observed (score 0) and diffuse cytoplasmic positivity for PTK7.
