H00002246-M01

antibody from Abnova Corporation

Targeting: FGF1 AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002246-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002246-M01, RRID:AB_463870

Product name

FGF1 monoclonal antibody (M01), clone 3F5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant FGF1.

Antigen sequence

VDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYL
AMDTDGLLYGSQTPNEECLFLERLEENHYNTYISK
KHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPL
PVSSD


Isotype

IgG

Antibody clone number

3F5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Acidic fibroblast growth factor (FGF) potentiates glial-mediated neurotoxicity by activating FGFR2 IIIb protein.

Lee M, Kang Y, Suk K, Schwab C, Yu S, McGeer PL
The Journal of biological chemistry 2011 Dec 2;286(48):41230-45

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to FGF1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between FGFR2 and FGF1. HeLa cells were stained with anti-FGFR2 rabbit purified polyclonal 1:1200 and anti-FGF1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)