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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA319569 - Provider product page
- Provider
- OriGene
- Product name
- Mouse monoclonal anti-HEF1 antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal anti-HEF1 antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NEDD9
- Antibody clone number
- 14A11
- Vial size
- 100 µg
- Concentration
- 1 mg/ml
Submitted references Altered function of the glutamate-aspartate transporter GLAST, a potential therapeutic target in glioblastoma.
Corbetta C, Di Ianni N, Bruzzone MG, Patanè M, Pollo B, Cantini G, Cominelli M, Zucca I, Pisati F, Poliani PL, Finocchiaro G, Pellegatta S
International journal of cancer 2019 May 15;144(10):2539-2554
International journal of cancer 2019 May 15;144(10):2539-2554
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blotting using Monoclonal anti-HEF1 antibody (clone 14A11) shows detection of HEF1 present in MCF-7 cells induced to express HEF1 by tetracycline removal (right lane). See Pugacheva et al for details.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence microscopy using Monoclonal anti-HEF1 antibody (clone 14A11) shows detection of HEF1 localized at the centrosome (bright dots) and focal adhesion sites. The antibody was used at a 1:100 dilution with a 1-min exposure time. Personal Communication. Elena Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.
- Validation comment
- IF
