Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX116033 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116033, RRID:AB_2036854
- Product name
- Endothelin 1 antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references EZH2, Endothelin-1, and CD34 as Biomarkers of Aggressive Cervical Squamous Cell Carcinoma: An Immunohistochemical Study.
Endothelin-1 receptor blockade as new possible therapeutic approach in multiple myeloma.
Endothelin-1 and ET receptors impair left ventricular function by mediated coronary arteries dysfunction in chronic intermittent hypoxia rats.
Evidence for proangiogenic cellular and humoral systemic response in patients with acute onset of spinal cord injury.
Overexpression of endothelin 1 triggers hepatocarcinogenesis in zebrafish and promotes cell proliferation and migration through the AKT pathway.
Identification of the common regulators for hepatocellular carcinoma induced by hepatitis B virus X antigen in a mouse model.
Fathy A, Abdelrahman AE
Turk patoloji dergisi 2018;34(2):150-57
Turk patoloji dergisi 2018;34(2):150-57
Endothelin-1 receptor blockade as new possible therapeutic approach in multiple myeloma.
Russignan A, Spina C, Tamassia N, Cassaro A, Rigo A, Bagnato A, Rosanò L, Bonalumi A, Gottardi M, Zanatta L, Giacomazzi A, Scupoli MT, Tinelli M, Salvadori U, Mosna F, Zamò A, Cassatella MA, Vinante F, Tecchio C
British journal of haematology 2017 Sep;178(5):781-793
British journal of haematology 2017 Sep;178(5):781-793
Endothelin-1 and ET receptors impair left ventricular function by mediated coronary arteries dysfunction in chronic intermittent hypoxia rats.
Wang JW, Li AY, Guo QH, Guo YJ, Weiss JW, Ji ES
Physiological reports 2017 Jan;5(1)
Physiological reports 2017 Jan;5(1)
Evidence for proangiogenic cellular and humoral systemic response in patients with acute onset of spinal cord injury.
Paczkowska E, Rogińska D, Pius-Sadowska E, Jurewicz A, Piecyk K, Safranow K, Dziedziejko V, Grzegrzółka R, Bohatyrewicz A, Machaliński B
The journal of spinal cord medicine 2015 Nov;38(6):729-44
The journal of spinal cord medicine 2015 Nov;38(6):729-44
Overexpression of endothelin 1 triggers hepatocarcinogenesis in zebrafish and promotes cell proliferation and migration through the AKT pathway.
Lu JW, Liao CY, Yang WY, Lin YM, Jin SL, Wang HD, Yuh CH
PloS one 2014;9(1):e85318
PloS one 2014;9(1):e85318
Identification of the common regulators for hepatocellular carcinoma induced by hepatitis B virus X antigen in a mouse model.
Lu JW, Hsia Y, Yang WY, Lin YI, Li CC, Tsai TF, Chang KW, Shieh GS, Tsai SF, Wang HD, Yuh CH
Carcinogenesis 2012 Jan;33(1):209-19
Carcinogenesis 2012 Jan;33(1):209-19
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: Hep G2 (GTX27900) 12% SDS PAGE GTX116033 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Endothelin 1 antibody detects Endothelin 1 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Endothelin 1 antibody (GTX116033) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with Endothelin 1 antibody [C1C3] (GTX116033) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with Endothelin 1 antibody [C1C3] (GTX116033) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Endothelin 1 antibody [C1C3] detects Endothelin 1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Endothelin 1 protein stained by Endothelin 1 antibody [C1C3] (GTX116033) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.