GTX22786

antibody from GeneTex

Targeting: EDN1 ET1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Radioimmunoassay (Data presented on provider website)

Antibody Data

Product number

GTX22786

Provider

GeneTex

Proper citation

GeneTex Cat#GTX22786, RRID:AB_384849

Product name

Endothelin 1 antibody [TR.ET.48.5]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Canine, Porcine, Sheep

Host

Mouse

References

Submitted references

Increased tissue endothelin-1 and endothelin-B receptor expression in temporal arteries from patients with giant cell arteritis.

Dimitrijevic I, Andersson C, Rissler P, Edvinsson L
Ophthalmology 2010 Mar;117(3):628-36

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of Endothelin 1 in 25 ug of PC12 cell lysates. Proteins were transferred to a PVDF membrane and blocked at 4¢XC overnight. The membrane was probed with Endothelin 1 antibody [TR.ET.48.5] at a dilution of 1:500 overnight at 4¢XC, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of Endothelin 1 (green) in HeLa cells compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with Endothelin 1 antibody [TR.ET.48.5] in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4¢XC in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometry analysis of Endothelin 1 in HepG2 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with Endothelin 1 antibody [TR.ET.48.5] at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a proper secondary antibody, and re-suspended in PBS for FACS analysis.