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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP51365PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51365PU-N, RRID:AB_11146877
- Product name
- anti Endothelin-1 (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 183-211 amino acids from the C-terminal region of Human EDN1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Endothelin-1 Antibody (C-term) Cat.-No AP51365PU-N in ZR-75-1, U251, MDA-MB453, A549 cell line lysates (35ug/lane). This demonstrates the EDN1 antibody detected the EDN1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Endothelin-1 Antibody (C-term) Cat.-No AP51365PU-N with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human liver tissue reacted with Endothelin-1 Antibody (C-term) Cat.-No AP51365PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
