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- Antibody Data
- Antigen structure
- References [2]
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- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- MAB3440 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Endothelin-1 C-Terminus Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Endothelin-1 C-Terminus. Recognizes the C-terminal region of mature Endothelin-1, -2, and -3 (amino acid residues 15-21).
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 3G10
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Increased mortality with elevated plasma endothelin-1 in acute heart failure: an ASCEND-HF biomarker substudy.
Expression of endothelial nitric oxide synthase and endothelin-1 in skin tissue from amputated limbs of patients with complex regional pain syndrome.
Perez AL, Grodin JL, Wu Y, Hernandez AF, Butler J, Metra M, Felker GM, Voors AA, McMurray JJ, Armstrong PW, Starling RC, O'Connor CM, Tang WH
European journal of heart failure 2016 Mar;18(3):290-7
European journal of heart failure 2016 Mar;18(3):290-7
Expression of endothelial nitric oxide synthase and endothelin-1 in skin tissue from amputated limbs of patients with complex regional pain syndrome.
Groeneweg JG, Antonissen CH, Huygen FJ, Zijlstra FJ
Mediators of inflammation 2008;2008:680981
Mediators of inflammation 2008;2008:680981
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human Endothelin-1 ELISA Standard Curve. Recombinant Human Endothelin-1 protein was serially diluted 2-fold and captured by Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB34401) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Endothelin-1 in HUVEC Human Cells. Endothelin-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
