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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- EB08990 - Provider product page
- Provider
- Everest Biotech
- Proper citation
- Everest Biotech Cat#EB08990, RRID:AB_2157742
- Product name
- OXTR
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-RRLGETSASKKSN- Isotype
- IgG
- Vial size
- 100ug
- Concentration
- 0.5mg/1ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB08990 (1µg/ml) staining of lysates of PC3 cells treated in several ways (50,000 cells per lane). Reprobed for alpha-tubulin (50kDa) to serve as loading control (OXTR antibody incubation was overnight. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB08990 (5µg/ml) staining of PC3 cells. Detected by DAB.
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB08990 (5µg/ml) staining of Human Prostate stromal cells. Overnight incubation at 4C and detected with Alexafluor secondary conjugated antibody.
