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- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- ABIN569580 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Oxytocin Receptor (OXTR) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide with sequence from the C-Terminus of the protein sequence according to NP_000907.2. Genename: OXTR
- Description
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-RRLGETSASKKSN- Epitope
- C-Term
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Hypothalamic oxytocin mRNA expression and melancholic depression.
Meynen G, Unmehopa UA, Hofman MA, Swaab DF, Hoogendijk WJ
Molecular psychiatry 2007 Feb;12(2):118-9
Molecular psychiatry 2007 Feb;12(2):118-9
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP22376PU-N OXTR antibody staining (1 μg/ml) of lysates of PC3 cells treated in several ways (50,000 cells per lane). Reprobed for alpha-tubulin (50kDa) to serve as loading control (OXTR antibody incubation was overnight. Detected by chemiluminescence.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP22376PU-N OXTR antibody staining of Rat Uterus (U) and Rat Skeletal Muscle (M) lysates at at 0.5 μg/ml (35μg protein in RIPA buffer). Data obtained from Maree Gould, University of Otago, New Zealand. Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP22376PU-N OXTR antibody staining of PC3 cells at 5μg/ml. Detected by DAB.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP22376PU-N OXTR antibody staining of Human Prostate stromal cells. Overnight incubation at 4°C and detected with Alexa Fluor secondary conjugated antibody.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- AP22376PU-N OXTR antibody staining of Paraffin Embedded Human Prostate cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
