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Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA1-31714 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNMT3L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-31714 detects Dnmt3L from human samples.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1.11 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNMT3L in transfected U2OS cells (10cm dish, using 3 µg of GAL4-DNMT3L and protein extraction using IPH at 150mM) using a DNMT3L polyclonal antibody (Product # PA1-31714) at a dilution of 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DNMT3L was performed using DNMT3L Polyclonal Antibody (Product # PA1-31714) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNMT3L Polyclonal Antibody (Product # PA1-31714) was used at a 1:1,500 dilution to detect human DNMT3L by western blot after immunoprecipitation using the same antibody. Transfection of U2OS cells (10cm dish) was accomplished using 3 µg of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For IP and Western Blot conditions see Fuks et al. (2000). For western blotting the antibody was used at 0.4 µg/mL in TBS milk 2%, BSA 0.5%. Detection occurred using ECL. Note: this antibody works for western blotting only following immunoprecipitation. We have not had good success using the antibody directly for western blotting. The expected molecular weight of human DNMT3L is 43.6 kDa.
