H00029947-D01

antibody from Abnova Corporation

Targeting: DNMT3L MGC1090

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00029947-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00029947-D01, RRID:AB_10720105

Product name

DNMT3L MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human DNMT3L protein.

Antigen sequence

MAAIPALDPEAEPSMDVILVGSSELSSSVSPGTGR
DLIAYEVKANQRNIEDICICCGSLQVHTQHPLFEG
GICAPCKDKFLDALFLYDDDGYQSYCSICCSGETL
LICGNPDCTRCYCFECVDSLVGPGTSGKVHAMSNW
VCYLCLPSSRSGLLQRRRKWRSQLKAFYDRESENP
LEMFETVPVWRRQPVRVLSLFEDIKKELTSLGFLE
SGSDPGQLKHVVDVTDTVRKDVEEWGPFDLVYGAT
PPLGHTCDRPPSWYLFQFHRLLQYARPKPGSPGPF
FWMFVDNLVLNKEDLDVASRFLEMEPVTIPDVHGG
SLQNAVRVWSNIPAIRSRHWALVSEEELSLLAQNK
QSSKLAAKWPTKLVKNCFLPLREYFKYFSTELTSS
L


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DNMT3L MaxPab rabbit polyclonal antibody. Western Blot analysis of DNMT3L expression in mouse liver.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DNMT3L expression in transfected 293T cell line (H00029947-T02) by DNMT3L MaxPab polyclonal antibody.Lane 1: DNMT3L transfected lysate(43.5 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to DNMT3L on HeLa cell. [antibody concentration 30 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of DNMT3L transfected lysate using anti-DNMT3L MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DNMT3L purified MaxPab mouse polyclonal antibody (B01P) (H00029947-B01P).

Validation comment

Immunoprecipitation

Protocol

Protocol