AP16040PU-N

antibody from Acris Antibodies GmbH

Targeting: AIRE APECED, APS1, PGA1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

AP16040PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP16040PU-N, RRID:AB_1928009

Product name

anti AIRE / APECED (Isoform 1+2) (C-term)

Antibody type

Polyclonal

Antigen

Peptide from C-Terminus of the protein sequence according to NP_000374.1, NP_000649.1.

Reactivity

Human

Host

Goat

Vial size

0.1 mg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AIRE Antibody staining (AP16040PU-N at 0.01µg/ml) of HEK293 cell lysate. Lane 1: Untransfected and Lane 2: transfected with Human AIRE. Data kindly provided by Prof. PÌ_rt Peterson, University of Tartu, Estonia.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Staining of Human Lymph lysate using AIRE Antibody Cat.-No AP16040PU-N at 0.5 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AIRE Antibody (AP16040PU-N at 2.5 µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Paraffin embedded Human Thymus stained with AIRE Antibody Cat.-No AP16040PU-N at 2 µg/ml. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.