Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R35463 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- AIRE Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids QSMARPAAPFPS were used as the immunogen for this AIRE antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the AIRE antibody at -20°C.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human lymph node lysate with AIRE antibody at 0.5ug/ml. Predicted molecular weight: ~58/37kDa (isoforms 1/2). Both observed bands are both blocked by the immunizing peptide.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of untransfected (lane 1) and transfected (lane 2) HEK293 cell lysates with AIRE antibody at 0.05ug/ml.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human thymus with AIRE antibody at 2ug/ml. HIER: steamed with pH9 Tris/EDTA buffer, HRP-staining.
