PAB23255

antibody from Abnova Corporation

Targeting: AIRE APECED, APS1, PGA1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23255

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23255, RRID:AB_11121268

Product name

AIRE polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant AIRE.

Antigen sequence

PEDKFQETLHLKEKEGCPQAFHALLSWLLTQDSTA
ILDFWRVLFKDYNLERYGRLQPILDSFPKDVDLSQ


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with AIRE polyclonal antibody (Cat # PAB23255) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with AIRE polyclonal antibody (Cat # PAB23255) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, plasma membrane, cytoplasm and cytoskeleton (actin filaments).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with AIRE polyclonal antibody (Cat # PAB23255) shows strong cytoplasmic positivity in glandular cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)