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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NB100-1063 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1063, RRID:AB_10003404
- Product name
- Goat Polyclonal Autoimmune Regulator/AIRE Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The autoimmune regulator (AIRE) is a DNA-binding protein.
Kumar PG, Laloraya M, Wang CY, Ruan QG, Davoodi-Semiromi A, Kao KJ, She JX
The Journal of biological chemistry 2001 Nov 2;276(44):41357-64
The Journal of biological chemistry 2001 Nov 2;276(44):41357-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Staining of nuclear Jurkat cell with antibody at 1 ug/mL (A), Human Spleen with antibody at 2 ug/mL (B) and negative control Human Smooth Muscle (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Antibody (2 ug/mL) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Autoimmune Regulator/AIRE Antibody [NB100-1063] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
