PAB4825

antibody from Abnova Corporation

Targeting: SIRT5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB4825

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB4825, RRID:AB_1580647

Product name

SIRT5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of SIRT5.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Phylogenetic classification of prokaryotic and eukaryotic Sir2-like proteins.

Frye RA
Biochemical and biophysical research communications 2000 Jul 5;273(2):793-8

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Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity.

Frye RA
Biochemical and biophysical research communications 1999 Jun 24;260(1):273-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of SIRT5 polyclonal antibody (Cat # PAB4825) in Jurkat cell lysate (35 ug/lane). SIRT5 (arrow) was detected using the purified polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SIRT5 polyclonal antibody (Cat # PAB4825) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)