15122-1-AP

antibody from Invitrogen Antibodies

Targeting: SIRT5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15122-1-AP

Provider

Invitrogen Antibodies

Product name

SIRT5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MRPLQIVPS RLISQLYCGL KPPASTRNQI CLKMARPSSS MADFRKFFAK AKHIVIISGA GVSAESGVPT FRGAGGYWRK WQAQDLATPL AFAHNPSRVW EFYHYRREVM GSKEPNAGHR AIAECETRLG KQGRRVVVIT QNIDELHRKA GTKNLLEIHG SLFKTRCTSC GVVAENYKSP ICPALSGKGA PEPGTQDASI PVEKLPRCEE AGCGGLLRPH VVWFGENLDP AILEEVDREL AHCDLCLVVG TSSVVYPAAM FAPQVAARGV PVAEFNTETT PATNRFRFHF QGPCGTTLPE ALACHENETV S (1-310 aa encoded by BC000126)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15122-1-AP (SIRT5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15122-1-AP (SIRT5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15122-1-AP (SIRT5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 15122-1-AP (SIRT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SIRT5 (IP:15122-1-AP, 4ug; Detection:15122-1-AP 1:500) with mouse liver tissue lysate 6000ug.