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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- GTX102229 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102229, RRID:AB_1952588
- Product name
- XBP1 antibody [N3C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Molt-4 (GTX27912) B: Raji 12% SDS PAGE GTX102229 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with XBP1 antibody [N3C3] (GTX102229) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody [N3C3] (GTX102229) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- XBP1 antibody [N3C3] detects XBP1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: XBP1 protein stained by XBP1 antibody [N3C3] (GTX102229) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, stained by () diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using XBP1(GTX102229) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- XBP1 antibody [N3C3] detects XBP1 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. XBP1 antibody [N3C3] (GTX102229) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- XBP1 antibody [N3C3] detects XBP1 protein at nucleus in rat ovary by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. XBP1 antibody [N3C3] (GTX102229) diluted at 1:500.
