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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB7873 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB7873, RRID:AB_10676726
- Product name
- XBP1 monoclonal antibody, clone 3H1G4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant XBP1.
- Antibody clone number
- 3H1G4
- Storage
- Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA.
XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor.
Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress.
Calfon M, Zeng H, Urano F, Till JH, Hubbard SR, Harding HP, Clark SG, Ron D
Nature 2002 Jan 3;415(6867):92-6
Nature 2002 Jan 3;415(6867):92-6
XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor.
Yoshida H, Matsui T, Yamamoto A, Okada T, Mori K
Cell 2001 Dec 28;107(7):881-91
Cell 2001 Dec 28;107(7):881-91
Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress.
Haze K, Yoshida H, Yanagi H, Yura T, Mori K
Molecular biology of the cell 1999 Nov;10(11):3787-99
Molecular biology of the cell 1999 Nov;10(11):3787-99
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of 100 ng of XBP1 recombinant protein with XBP1 monoclonal antibody, clone 3H1G4 (Cat # MAB7873) at 1 ug/mL .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemistry of XBP1 in HepG2 cells with XBP1 monoclonal antibody, clone 3H1G4 (Cat # MAB7873) at 2 ug/mL .
- Validation comment
- Immunocytochemistry
