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- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [1]
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- Product number
- 647501 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#647501, RRID:AB_2241744
- Product name
- Purified anti-XBP-1s
- Antibody type
- Monoclonal
- Antigen
- XBP-1s recombination protein
- Description
- XBP-1 is a transcription factor containing a bZIP domain. It was first identified because of its binding ability to X-box, a conserved transcriptional element in the promoter of human HLA DR gene. XBP-1 has multiple functions. It controls MHC class II gene regulation and is also essential for differentiation of plasma cells. XBP-1 upregulates as part of the ER stress response, also known as the unfolded protein response. Unspliced XBP-1 is 261 amino acids and migrates on the SDS-PAGE around 33 kD; spliced XBP-1, recognized by clone 143F, is 371 amino acids and migrates around 55 kD.?È????
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
Submitted references Targeting the UPR transcription factor XBP1 protects against Huntington's disease through the regulation of FoxO1 and autophagy.
MicroRNA-30c-2* limits expression of proadaptive factor XBP1 in the unfolded protein response.
Involvement of X-box binding protein 1 and reactive oxygen species pathways in the pathogenesis of tumour necrosis factor receptor-associated periodic syndrome.
The unfolded protein response in familial amyotrophic lateral sclerosis.
Bortezomib induction of C/EBPβ mediates Epstein-Barr virus lytic activation in Burkitt lymphoma.
Preconditioning with endoplasmic reticulum stress mitigates retinal endothelial inflammation via activation of X-box binding protein 1.
Mutant HFE H63D protein is associated with prolonged endoplasmic reticulum stress and increased neuronal vulnerability.
MAPK signaling to the early secretory pathway revealed by kinase/phosphatase functional screening.
Surviving endoplasmic reticulum stress is coupled to altered chondrocyte differentiation and function.
Vidal RL, Figueroa A, Court FA, Thielen P, Molina C, Wirth C, Caballero B, Kiffin R, Segura-Aguilar J, Cuervo AM, Glimcher LH, Hetz C
Human molecular genetics 2012 May 15;21(10):2245-62
Human molecular genetics 2012 May 15;21(10):2245-62
MicroRNA-30c-2* limits expression of proadaptive factor XBP1 in the unfolded protein response.
Byrd AE, Aragon IV, Brewer JW
The Journal of cell biology 2012 Mar 19;196(6):689-98
The Journal of cell biology 2012 Mar 19;196(6):689-98
Involvement of X-box binding protein 1 and reactive oxygen species pathways in the pathogenesis of tumour necrosis factor receptor-associated periodic syndrome.
Dickie LJ, Aziz AM, Savic S, Lucherini OM, Cantarini L, Geiler J, Wong CH, Coughlan R, Lane T, Lachmann HJ, Hawkins PN, Robinson PA, Emery P, McGonagle D, McDermott MF
Annals of the rheumatic diseases 2012 Jul 6;
Annals of the rheumatic diseases 2012 Jul 6;
The unfolded protein response in familial amyotrophic lateral sclerosis.
Wang L, Popko B, Roos RP
Human molecular genetics 2011 Mar 1;20(5):1008-15
Human molecular genetics 2011 Mar 1;20(5):1008-15
Bortezomib induction of C/EBPβ mediates Epstein-Barr virus lytic activation in Burkitt lymphoma.
Shirley CM, Chen J, Shamay M, Li H, Zahnow CA, Hayward SD, Ambinder RF
Blood 2011 Jun 9;117(23):6297-303
Blood 2011 Jun 9;117(23):6297-303
Preconditioning with endoplasmic reticulum stress mitigates retinal endothelial inflammation via activation of X-box binding protein 1.
Li J, Wang JJ, Zhang SX
The Journal of biological chemistry 2011 Feb 11;286(6):4912-21
The Journal of biological chemistry 2011 Feb 11;286(6):4912-21
Mutant HFE H63D protein is associated with prolonged endoplasmic reticulum stress and increased neuronal vulnerability.
Liu Y, Lee SY, Neely E, Nandar W, Moyo M, Simmons Z, Connor JR
The Journal of biological chemistry 2011 Apr 15;286(15):13161-70
The Journal of biological chemistry 2011 Apr 15;286(15):13161-70
MAPK signaling to the early secretory pathway revealed by kinase/phosphatase functional screening.
Farhan H, Wendeler MW, Mitrovic S, Fava E, Silberberg Y, Sharan R, Zerial M, Hauri HP
The Journal of cell biology 2010 Jun 14;189(6):997-1011
The Journal of cell biology 2010 Jun 14;189(6):997-1011
Surviving endoplasmic reticulum stress is coupled to altered chondrocyte differentiation and function.
Tsang KY, Chan D, Cheslett D, Chan WC, So CL, Melhado IG, Chan TW, Kwan KM, Hunziker EB, Yamada Y, Bateman JF, Cheung KM, Cheah KS
PLoS biology 2007 Mar;5(3):e44
PLoS biology 2007 Mar;5(3):e44
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Extracts of untreated HepG2 cells (Lane 1) and extracts of HepG2 cells treated with 300 nM thapsigargin (TG) for 16 hours (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-Xbp-1s antibody (clone 143F). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
