AF3377

antibody from Novus Biologicals

Targeting: IFITM3 1-8U, DSPA2b

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3377

Provider

Novus Biologicals

Product name

Goat Polyclonal IFITM3/Fragilis Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse IFITM3/Fragilis in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human IFITM3 is observed.

Reactivity

Mouse

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

OTX2 restricts entry to the mouse germline.

Zhang J, Zhang M, Acampora D, Vojtek M, Yuan D, Simeone A, Chambers I
Nature 2018 Oct;562(7728):595-599

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Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication.

Kuss-Duerkop SK, Wang J, Mena I, White K, Metreveli G, Sakthivel R, Mata MA, Muñoz-Moreno R, Chen X, Krammer F, Diamond MS, Chen ZJ, García-Sastre A, Fontoura BMA
PLoS pathogens 2017 Sep;13(9):e1006635

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IFITM1 suppresses expression of human endogenous retroviruses in human embryonic stem cells.

Fu Y, Zhou Z, Wang H, Gong P, Guo R, Wang J, Lu X, Qi F, Liu L
FEBS open bio 2017 Aug;7(8):1102-1110

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Interferon-induced transmembrane protein 3 is a type II transmembrane protein.

Bailey CC, Kondur HR, Huang IC, Farzan M
The Journal of biological chemistry 2013 Nov 8;288(45):32184-93

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Ifitm3 limits the severity of acute influenza in mice.

Bailey CC, Huang IC, Kam C, Farzan M
PLoS pathogens 2012 Sep;8(9):e1002909

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse IFITM3/Fragilis by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, D3 mouse embryonic stem cell line, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM3/Fragilis at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

IFITM3/Fragilis in Embryonic Mouse Ovary. IFITM3/Fragilis was detected in immersion fixed frozen sections of embryonic mouse ovary (E13.5) using 10 µg/mL Goat Anti-Mouse IFITM3/Fragilis Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3377) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.