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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00010410-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010410-M02, RRID:AB_10633828
- Product name
- IFITM3 monoclonal antibody (M02), clone 2H4-1D5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant IFITM3.
- Antigen sequence
MNHTVQTFFSPVNSGQPPNYEMLKEEHEVAVLGAP
HNPAPPTSTVIHIRSETSVPDHVVWSLFNTLFMNP
CCLGFIAFAYSVKSRDRKMVGDVTGAQAYASTAKC
LNIWALILGILMTILLIVIPVLIFQAYG- Isotype
- IgG
- Antibody clone number
- 2H4-1D5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of IFITM3 expression in transfected 293T cell line by IFITM3 monoclonal antibody (M02), clone 2H4-1D5.Lane 1: IFITM3 transfected lysate (Predicted MW: 14.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged IFITM3 is 0.03 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of IFITM3 transfected lysate using anti-IFITM3 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IFITM3 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
