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LearnACC-128-200UL
antibody from Invitrogen Antibodies
Targeting: TRPM2
EREG1, KNP3, LTRPC2, NUDT9H, NUDT9L1, TRPC7
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ACC-128-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRPM2 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4): - 1, 2. Anti-TRPM2 (extracellular) Antibody (#ACC-128), (1:200). 3, 4. Anti-TRPM2 (extracellular) Antibody , preincubated with TRPM2 (extracellular) Blocking Peptide (#BLP-CC128).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4): - 1, 2. Anti-TRPM2 (extracellular) Antibody (#ACC-128), (1:200). 3, 4. Anti-TRPM2 (extracellular) Antibody , preincubated with TRPM2 (extracellular) Blocking Peptide (#BLP-CC128).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of TRPM2 in rat substantia nigra - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM2 (extracellular) Antibody (#ACC-128), followed by goat Anti-rabbit-AlexaFluor-488. TRPM2 immunoreactivity (green) appears in neurons in the substantia nigra pars compacta (SNC), (arrows). SNR=substantia nigra pars reticulata. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of TRPM2 in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-TRPM2 (extracellular) Antibody (#ACC-128), (2.5 µg) + goat- Anti-rabbit-FITC.
