TA322140

antibody from OriGene

Targeting: KISS1R AXOR12, GPR54, HOT7T175

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA322140

Provider

OriGene

Product name

Anti-KISS1R Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to a region derived from 383-398 amino acids of Human KISS1 receptor

Description

Anti-KISS1R Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

0.5mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 43 kDa . Positive control: Jurkat, Hela, 231 cell and Mouse pancreas tissue lysate. Recommended dilution: 1/200-1000. (Gel: 12%SDS-PAGE Lane1: Jurkat cell lysate Lane2: Hela cell lysate Lane3: 231 cell lysate Lane4: Mouse pancreas tissue lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/15-50 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using KISS1R antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC