Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-49719 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KISS1R Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous level of total KISS1R / GPR54 protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KISS1R was performed by loading Lane1: Jurkat cell lysate (40µg), Lane2: Hela cell lysate (40µg), Lane3: 231 cell lysate (40µg), Lane4: Mouse pancreas tissue lysate (40µg) on to a 12% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a KISS1R antibody (Product # PA5-49719) at a 1/500 dilution for 10 minutes, followed by Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of KISS1R in Hela cells. The cells were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 °C. Cells were incubated with KISS1R Polyclonal Antibody (Product # PA5-49719) at a 1:200 dilution for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1:600, was used as the secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of KISS1R in paraffin-embedded human lung cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with KISS1R Polyclonal Antibody (Product # PA5-49719) at a 1:100 dilution at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of KISS1R in paraffin-embedded Rat kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with KISS1R Polyclonal Antibody (Product # PA5-49719) at a 1:100 dilution at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of KISS2R in paraffin-embedded Mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with KISS1R Polyclonal Antibody (Product # PA5-49719) at a 1:100 dilution at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.