Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-20372 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STIM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is A-20 cell lysate. PA5-20372 can be used with blocking peptide PEP-0489.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Furin Prodomain ppFurin Enhances Ca(2+) Entry Through Orai and TRPC6 Channels' Activation in Breast Cancer Cells.
López JJ, Siegfried G, Cantonero C, Soulet F, Descarpentrie J, Smani T, Badiola I, Pernot S, Evrard S, Rosado JA, Khatib AM
Cancers 2021 Apr 1;13(7)
Cancers 2021 Apr 1;13(7)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of A-20 cell lysate using a STIM2 polyclonal antibody (Product # PA5-20372) at (A) 0.5 and (B) 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of STIM2 in A-20 cell lysate with STIM2 Polyclonal Antibody (Product # PA5-20372) at (A) 0.5 and (B) 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of human spleen cells using a STIM2 polyclonal antibody (Product # PA5-20372) at a 20 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of STIM2 in Human Spleen cells with STIM2 Polyclonal Antibody (Product # PA5-20372) at 20 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of STIM2 in human spleen tissue with STIM2 Polyclonal Antibody (Product # PA5-20372) at 2.5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 ppFurin enhances store-operated Ca 2+ entry (SOCE) in MDA-MB-231 cells. ( a ) Control MDA-MB-231 and MDA-MB-435 cells or expressing ppFurin were lysed and whole cell lysates were analyzed by Western blotting using anti-Orai1, Orai3, STIM1, STIM2, TRPC1, TRPC6, or beta-actin antibody, as indicated. Blot images are from one experiment representative of three which gave similar results. ( b - e ). Changes in [Ca 2+ ] I were detected in fura-2-loaded control MDA-MB-231 and MDA-MB-435 cells or expressing ppFurin. Cells were stimulated with 1 uM of TG in a Ca 2+ -free medium (100 uM of EGTA added), and later SOCE was detected by the addition of 1 mM of CaCl 2 to the extracellular medium. ( b , c ) Traces are representative of 40 cells/day/3-5 days. ( d , e ) Histograms indicate TG-induced Ca 2+ release and SOCE as the area under the curve, expressed as mean +- SEM and presented as percentage of their respective control cells. Statistical significance was assessed by Student''s t -test and * represents p < 0.05 as compared to cells transfected with pIRES2-EGFP empty vector.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 ppFurin induces constitutive Ca 2+ entry in MDA-MB-231 cells. ( a - f ) Changes in [Ca 2+ ] I were detected in fura-2-loaded control MDA-MB-231 and MDA-MB-435 and MCF7 cells or expressing ppFurin. Cells were maintained in a Ca 2+ -free medium (100 uM of EGTA added), and later 1 mM of CaCl 2 was added in the extracellular medium to detect the existence of a constitutive Ca 2+ entry. Each trace is representative of 40 cells/day/3-5 days. Histograms represent constitutive Ca 2+ entry as the area under the curve, expressed as mean +- SEM and presented as the percentage of their respective control cells. Statistical significance was assessed by Student''s t -test and * represents p < 0.05 as compared to the corresponding control. ( g , h ) Control and ppFurin-expressing MDA-MB-231 cells were transfected with shTRPC6 or scramble plasmid, as indicated. TRPC6 and beta-actin detection was carried out at 48 h post-transfection by Western blotting as described in the Material and Methods section. Blot image is from one experiment representative of five which gave similar results. Histograms show TRPC6 expression represented as percentage of control and expressed as mean +- SEM. Statistical significance was assessed by one-way ANOVA combined with post-hoc Tukey''s test. * p < 0.05 as compared to the corresponding control. ( i - l ) Control and ppFurin-expressing MDA-MB-231 and MDA-MB-435 cells were transfected with shTRPC6 or scramble plasmid, as indicated. Loading cells with Fu