GTX26190

antibody from GeneTex

Targeting: ALDOA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX26190

Provider

GeneTex

Proper citation

GeneTex Cat#GTX26190, RRID:AB_374380

Product name

Aldolase A antibody

Antibody type

Polyclonal

Reactivity

Human, Rabbit

Host

Goat

References

Submitted references

Protection from oxidative stress-induced apoptosis in cortical neuronal cultures by iron chelators is associated with enhanced DNA binding of hypoxia-inducible factor-1 and ATF-1/CREB and increased expression of glycolytic enzymes, p21(waf1/cip1), and erythropoietin.

Zaman K, Ryu H, Hall D, O'Donovan K, Lin KI, Miller MP, Marquis JC, Baraban JM, Semenza GL, Ratan RR
The Journal of neuroscience : the official journal of the Society for Neuroscience 1999 Nov 15;19(22):9821-30

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IgG purified antibody to rabbit muscle aldolase (GTX26190) was used at a 1:1000 dilution to detect human aldolase by Western blot. A whole cell lysate prepared from human derived A293 cells was loaded on a 4-12% tris glycine gradient gel for SDS-PAGE. The gel was transferred to nitro-cellulose using standard techniques. Antibody reaction with the membrane occurred overnight at 4¢X C in TTBS supplemented with 2% non-fat dry milk. Color was allowed to develop using SuperSignal West Pico Chemiluminescent Substrate (PIERCE). Other detection methods will yield similar results. This antibody clearly detects a band at ~41 kDa consistent with human aldolase.