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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- R31844 - Provider product page
- Provider
- NSJ Bioreagents
- Proper citation
- NSJ Bioreagents Cat#R31844, RRID:AB_2725751
- Product name
- ABCB11 Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids KYGDNTKEIPMERVIAAAKQAQLHD of human ABCB11 were used as the immunogen for the ABCB11 antibody.
- Description
- Antigen affinity
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the ABCB11 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Submitted references Constitutive Androstane Receptor Differentially Regulates Bile Acid Homeostasis in Mouse Models of Intrahepatic Cholestasis
Xenobiotic Nuclear Receptor Signaling Determines Molecular Pathogenesis of Progressive Familial Intrahepatic Cholestasis
Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing
Kim K, Choi J, Li F, Dong B, Wooton-Kee C, Arizpe A, Anakk S, Jung S, Hartig S, Moore D
Hepatology Communications 2019 January;3(1):147-159
Hepatology Communications 2019 January;3(1):147-159
Xenobiotic Nuclear Receptor Signaling Determines Molecular Pathogenesis of Progressive Familial Intrahepatic Cholestasis
Kim K, Choi J, Li F, Arizpe A, Wooton-Kee C, Anakk S, Jung S, Finegold M, Moore D
Endocrinology 2018 June;159(6):2435-2446
Endocrinology 2018 June;159(6):2435-2446
Rapid Disruption of Genes Specifically in Livers of Mice Using Multiplex CRISPR/Cas9 Editing
Pankowicz F, Barzi M, Kim K, Legras X, Martins C, Wooton-Kee C, Lagor W, Marini J, Elsea S, Bissig-Choisat B, Moore D, Bissig K
Gastroenterology 2018 August
Gastroenterology 2018 August
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat liver, 2) mouse liver and 3) human SMMC lysate with ABCB11 antibody. Expected/observed molecular weight ~146 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human liver cancer with ABCB11 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse liver with ABCB11 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat liver with ABCB11 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A431 cells with ABCB11 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=ABCB11 antibody.
