10538-1-AP

antibody from Invitrogen Antibodies

Targeting: CAPN1 CANP, CANPL1, muCANP, muCL

Immunoprecipitation

Antibody data

Submit

Validation data

Product number: 10538-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: Calpain 1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: RESGCSFVL ALMQKHRRRE RRFGRDMETI GFAVYEVPPE LVGQPAVHLK RDFFLANASR ARSEQFINLR EVSTRFRLPP GEYVVVPSTF EPNKEGDFVL RFFSEKSAGT VELDDQIQAN LPDEQVLSEE EIDENFNALF RQLAGEDMEI SVKELRTILN RIISKHKDLR TKGFSLESCR SMVNLMDRDG NGKLGLVEFN ILWNRIRNYL SIFRKFDLDK SGSMSAYEMR MAIESAGFKL NKKLYELIIT RYSEPDLAVD FDNFVCCLVR LETMFRFFKT LDTDLDGVVT FDLFKWLQLT MFA (413-714 aa encoded by BC008751)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.35 mg/mL
Storage: -20°C

CAPN1 protein structure - 10538-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A2780 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: PC-3 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse testis tissue were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP (Calpain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human heart using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human heart using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human testis using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human testis using 10538-1-AP (Calpain 1 antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP ( Calpain 1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human urothelial carcinoma tissue slide using 10538-1-AP ( Calpain 1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).