10538-1-AP

antibody from Proteintech Group

Targeting: CAPN1 CANP, CANPL1, muCANP, muCL

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

10538-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10538-1-AP, RRID:AB_10858627

Product name

Calpain 1 antibody

Antibody type

Polyclonal

Description

KD/KO validated Calpain 1 antibody (Cat. #10538-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

miR‑140‑5p alleviates mouse liver ischemia/reperfusion injury by targeting CAPN1.

Yu Q, Chen S, Tang H, Yang H, Zhang J, Shi X, Li J, Guo W, Zhang S
Molecular medicine reports 2021 Sep;24(3)

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Astrocytic Kir4.1 regulates NMDAR/calpain signaling axis in lipopolysaccharide-induced depression-like behaviors in mice.

Song Z, Bian Z, Zhang Z, Wang X, Zhu A, Zhu G
Toxicology and applied pharmacology 2021 Oct 15;429:115711

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The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.

Kotani T, Takegaki J, Tamura Y, Kouzaki K, Nakazato K, Ishii N
Physiological reports 2021 May;9(9):e14842

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Inactivation of cysteine 674 in the sarcoplasmic/endoplasmic reticulum calcium ATPase 2 causes retinopathy in the mouse.

Liu G, Wu F, Wu H, Wang Y, Jiang X, Hu P, Tong X
Experimental eye research 2021 Jun;207:108559

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NCX3 alleviates ethanol-induced apoptosis of SK-N-SH cells via the elimination of intracellular calcium ions.

Xia Z, Wang C, Wang X, Yu H, Yao H, Shen H, Lan X, Wu X, Zhang G
Toxicology in vitro : an international journal published in association with BIBRA 2021 Apr;72:105104

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Over-activation of NMDA receptors promotes ABCA1 degradation and foam cell formation.

Cheng H, Cheng Q, Bao X, Luo Y, Zhou Y, Li Y, Hua Q, Liu W, Tang S, Feng D, Luo Z
Biochimica et biophysica acta. Molecular and cell biology of lipids 2020 Oct;1865(10):158778

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Elastic titin properties and protein quality control in the aging heart.

Salcan S, Bongardt S, Monteiro Barbosa D, Efimov IR, Rassaf T, Krüger M, Kötter S
Biochimica et biophysica acta. Molecular cell research 2020 Mar;1867(3):118532

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CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.

Chen Y, Tang J, Lu T, Liu F
Thoracic cancer 2020 Jul;11(7):1848-1860

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Astragaloside IV protects against hyperglycemia-induced vascular endothelial dysfunction by inhibiting oxidative stress and Calpain-1 activation.

Nie Q, Zhu L, Zhang L, Leng B, Wang H
Life sciences 2019 Sep 1;232:116662

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Suppressing the Na(+)/H(+) exchanger 1: a new sight to treat depression.

Deng X, Ji Z, Xu B, Guo L, Xu L, Qin T, Feng L, Ma Z, Fu Q, Qu R, Quo Q, Ma S
Cell death & disease 2019 May 8;10(5):370

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Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Wang S, Huang Y, Yan Y, Zhou H, Wang M, Liao L, Wang Z, Chen D, Ji D, Xia X, Liu F, Huang J, Xiong K
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2019 Jan;221:57-67

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Triptolide protects against TGF-β1-induced pulmonary fibrosis by regulating FAK/calpain signaling.

Zhang P, Liu J, Zong R
Experimental and therapeutic medicine 2019 Dec;18(6):4781-4789

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Expression of Cell Cycle-related Proteins p16, p27 and Ki-67 Proliferating Marker in Laryngeal Squamous Cell Carcinomas and in Laryngeal Papillomas.

Ciesielska U, Zatonski T, Nowinska K, Ratajczak-Wielgomas K, Grzegrzolka J, Piotrowska A, Olbromski M, Pula B, Podhorska-Okolow M, Dziegiel P
Anticancer research 2017 May;37(5):2407-2415

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Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.

Zhang J, Lu S, Zhou Y, Meng K, Chen Z, Cui Y, Shi Y, Wang T, He QY
Proteomics 2017 Jul;17(13-14)

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Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.

Kmiecik AM, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski M, Podhorska-Okolow M, Dziegiel P
PloS one 2015;10(5):e0124865

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Calpain Cleaves Most Components in the Multiple Aminoacyl-tRNA Synthetase Complex and Affects Their Functions.

Lei HY, Zhou XL, Ruan ZR, Sun WC, Eriani G, Wang ED
The Journal of biological chemistry 2015 Oct 23;290(43):26314-27

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 10538-1-AP(CAPN1 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using CAPN1 antibody 10538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 10538-1-AP(CAPN1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human testis using 10538-1-AP(CAPN1 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue