HPA005992

antibody from Atlas Antibodies

Targeting: CAPN1 CANP, CANPL1, muCANP, muCL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA005992

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA005992, RRID:AB_1845924

Product name

Anti-CAPN1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

PDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPA
TFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALM
QKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVH
LKRDFFLANASRARSEQFINLREVSTRFRLPPGEY
VVVPSTFEPNKE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line A-431.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human fallopian tube and testis tissues using Anti-CAPN1 antibody. Corresponding CAPN1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows low expression as expected.

Sample type

HUMAN