15690-1-AP

antibody from Invitrogen Antibodies

Targeting: AP1B1 ADTB1, AP105A, BAM22, CLAPB2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15690-1-AP

Provider

Invitrogen Antibodies

Product name

AP2B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GSTDAGDSP VGTTTATNLE QPQVIPSQGD LLGDLLNLDL GPPVNVPQVS SMQMGAVDLL GGGLDSLLGS DLGGGIGGSP AVGQSFIPSS VPATFAPSPT PAVVSSGLND LFELSTGIGM APGGYVAPKA VWLPAVKAKG LEISGTFTHR QGHIYMEMNF TNKALQHMTD FAIQFNKNSF GVIPSTPLAI HTPLMPNQSI DVSLPLNTLG PVMKMEPLNN LQVAVKNNID VFYFSCLIPL NVLFVEDGKM ERQVFLATWK DIPNENELQF QIKECHLNAD TVSSKLQNNN VYTIAKRNVE GQDMLYQSLK LTNGIWILAE LRIQPGNPNY TLSLKCRAPE VSQYIYQVYD SILKN (598-951 aa encoded by BC006201)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 15690-1-AP (AP2B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

C6 cells were subjected to SDS PAGE followed by western blot with 15690-1-AP (AP2B1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15690-1-AP ( AP2B1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 15690-1-AP) (AP2B1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using AP2B1 antibody 15690-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-AP2B1 (IP:15690-1-AP, 4ug; Detection:15690-1-AP 1:500) with K-562 cells lysate 3200ug.