PAB2464

antibody from Abnova Corporation

Targeting: TXNL1 TRP32, Txl, TXNL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB2464

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB2464, RRID:AB_1581542

Product name

TXNL1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of TXNL1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Molecular cloning and expression of a cDNA encoding a human thioredoxin-like protein.

Miranda-Vizuete A, Gustafsson JA, Spyrou G
Biochemical and biophysical research communications 1998 Feb 4;243(1):284-8

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The TXNL1 polyclonal antibody (Cat # PAB2464) is used in Western blot to detect TXNL1 in HL-60 cell lysate.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Mouse cerebellar cortex showing molecular cell layer (mc), Purkinje cells (Pc) and granular cell layer.TXNL1 polyclonal antibody (Cat # PAB2464) gives strong nuclear labeling in Purkinje cells and granular cells with lower cytoplasmic staining.The results correspond precisely to TXNL1 mRNA expression shown with in situ hybridization (JimAcnez A et al. FEBS Lett. 2006, 580(3):960-7) and could be abolished with peptide used for immunization (Blocking peptide, Cat # P0347).The fixation was 4% paraformaldehyde perfusion (4mins) and 60 mins immersion. Staining was done in frozen sections with Vector Elite kit and nickel intensified DAB as a chromogen.Data courtesy of Prof. Markku Pelto-Huikko, Department of Developmental Biology, Medical School,Tampere University, Finland.

Validation comment

Immunohistochemistry (Frozen sections)