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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00010253-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010253-M01, RRID:AB_2195296
- Product name
- Mouse Monoclonal SPRY2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SPRY2 - sprouty homolog 2 (Drosophila)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references SPROUTY2 is a β-catenin and FOXO3a target gene indicative of poor prognosis in colon cancer.
Initial report on differential expression of sprouty proteins 1 and 2 in human epithelial ovarian cancer cell lines.
SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity.
Ordóñez-Morán P, Irmisch A, Barbáchano A, Chicote I, Tenbaum S, Landolfi S, Tabernero J, Huelsken J, Muñoz A, Pálmer HG
Oncogene 2014 Apr 10;33(15):1975-85
Oncogene 2014 Apr 10;33(15):1975-85
Initial report on differential expression of sprouty proteins 1 and 2 in human epithelial ovarian cancer cell lines.
Moghaddam SM, Amini A, Wei AQ, Pourgholami MH, Morris DL
Journal of oncology 2012;2012:373826
Journal of oncology 2012;2012:373826
SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity.
Barbáchano A, Ordóñez-Morán P, García JM, Sánchez A, Pereira F, Larriba MJ, Martínez N, Hernández J, Landolfi S, Bonilla F, Pálmer HG, Rojas JM, Muñoz A
Oncogene 2010 Aug 26;29(34):4800-13
Oncogene 2010 Aug 26;29(34):4800-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody (M01), clone 1E10.Lane 1: SPRY2 transfected lysate(34.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPRY2 Antibody (1e10) [H00010253-M01] - SPRY2 monoclonal antibody (M01), clone 1E10 Analysis of SPRY2 expression in C32.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SPRY2 Antibody (1e10) [H00010253-M01] - Detection limit for recombinant GST tagged SPRY2 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of monoclonal antibody to SPRY2 on HeLa cell. Antibody concentration 25 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SPRY2 Antibody (1e10) [H00010253-M01] - Analysis of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 3 ug/ml.
