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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- R1583P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1583P, RRID:AB_1007435
- Product name
- anti Sprouty homolog 2 / SPRY2
- Antibody type
- Polyclonal
- Antigen
- His x6 tagged full length human Sprouty-2 protein.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.37 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 2. Immunoprecipitation of HA-tagged hSpry2 using affinity purified anti-hSpry2 followed by immunoblot detection using monoclonal anti-HA. Lysates from Hela cells with overexpression of HA-hSpry2 (lane 1) and with no expression (lane 2) were probed. Personnel Communication. Kuldeep Patel, Loyola University.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blotting using anti-Sprouty-2. HeLa (lane 1) and SW13 (lane 2) cell lysates (30 µg) were separated by 10% SDS-PAGE and transferred to nitrocellulose membrane. The membrane was blocked with 5% milk/TBST for 1 h at room temperature followed by incubation with affinity purified anti-hSpry2 overnight at 4 ¡ C diluted 1:250 in blocking solution. The membrane was washed 3X with TBST and then incubated with a 1:5000 dilution of HRP Goat-a-Rabbit IgG diluted in blocking buffer for 1 h at room temperature. After final washes the proteins reactive on the membrane were detected using ECL. Other detection systems will yield similar results. Personnel Communication. Kuldeep Patel, Loyola University. Figure 2 - Immunoprecipitation of HA-tagged hSpry2 using affinity purified anti-hSpry2 followed by immunoblot detection using monoclonal anti-HA. Lysates from Hela cells with overexpression of HA-hSpry2 (lane 1) and with no expression (lane 2) were probed. Personnel Communication. Kuldeep Patel, Loyola University.
