15296-1-AP

antibody from Invitrogen Antibodies

Targeting: CARS1 CARS

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

15296-1-AP

Provider

Invitrogen Antibodies

Product name

CARS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MADSSGQQG KGRRVQPQWS PPAGTQPCRL HLYNSLTRNK EVFIPQDGKK VTWYCCGPTV YDASHMGHAR SYISFDILRR VLKDYFKFDV FYCMNITDID DKIIKRARQN HLFEQYREKR PEAAQLLEDV QAALKPFSVK LNETTDPDKK QMLERIQHAV QLATEPLEKA VQSRLTGEEV NSCVEVLLEE AKDLLSDWLD STLGCDVTDN SIFSKLPKFW EGDFHRDMEA LNVLPPDVLT RVSEYVPEIV NFVQKIVDNG YGYVSNGSVY FDTAKFASSE KHSYGKLVPE AVGDQKALQE GEGDLSISAD RLSEKRSPND FALWKASKPG EPSWPCPWGK GRPGWHIECS AMAG (1-353 aa encoded by BC002880)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.47 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15296-1-AP (CARS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were subjected to SDS PAGE followed by western blot with 15296-1-AP (CARS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15296-1-AP (CARS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15296-1-AP (CARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using CARS antibody 15296-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 15296-1-AP (CARS antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 15296-1-AP (CARS antibody) at dilution of 1:100 (under 40x lens).