MA5-37518

antibody from Invitrogen Antibodies

Targeting: IFNA2 IFN-alphaA, IFNA

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-37518

Provider

Invitrogen Antibodies

Product name

IFN alpha Monoclonal Antibody (278CT1)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

278CT1

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Defining the Innate Immune Responses for SARS-CoV-2-Human Macrophage Interactions.

Abdelmoaty MM, Yeapuri P, Machhi J, Olson KE, Shahjin F, Kumar V, Zhou Y, Liang J, Pandey K, Acharya A, Byrareddy SN, Mosley RL, Gendelman HE
Frontiers in immunology 2021;12:741502

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Defining the Immune Responses for SARS-CoV-2-Human Macrophage Interactions.

Abdelmoaty M, Yeapuri P, Machhi J, Olson K, Shahjin F, Zhou Y, Jingjing L, Pandey K, Acharya A, Byrareddy S, Mosley L, Gendelman H
bioRxiv : the preprint server for biology 2021 Jul 15;

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IFN alpha using the IFN alpha Monoclonal Antibody (Product # MA5-37518) in IFN cell line lysates (35 µg/lane).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 SARS-CoV-2 does not induce IFN activity in monocytes-macrophages. (A) MDMs were infected with SARS-CoV-2 (MOI=0.01) and the culture supernatants were collected at days 1, 3, and 5 post-infection. The collected culture supernatants were used to assess IFN activity in VSV-challenged bovine MDBK cells. Recombinant human IFN-alpha was used as standard (64 U/mul). 2-fold dilution was used for different conditions. ( A. i) Experimental timeline. ( A. ii) Representative images of IFN activity assay plates. (B) No additive or synergistic responses in poly(I:C)-induced IFN activity between control and SARS-CoV-2-challenged monocytes. Suspended monocytes were treated with 100 mug/ml poly(I:C) overnight before or after the infection with SARS-CoV-2 (MOI=0.01) for 4 h. ( B. i) Experimental timeline. (B .ii ) Representative images of IFN activity assay plates. (C) Western blot analysis was performed to determine expression of IFN-alpha and IFN-beta in cell lysates at different times after viral exposure. Representative immunoblot and densitometric quantification are shown. All experiments were done at least twice, and one representative image is shown. (C) Data represent mean +- SEM (n=3 donors). Statistical significance between the groups was determined with unpaired Student's t-test and p < 0.05 was considered significant.