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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Immunohistochemistry [3]
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- Product number
- NB120-6588 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-6588, RRID:AB_2083107
- Product name
- Rabbit Polyclonal Collagen VI alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- Reactivity
- Human, Mouse, Rat, Bovine, Feline
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Submitted references The role of extracellular matrix in mouse and human corneal neovascularization.
Genetic Deletion of Syndecan-4 Alters Body Composition, Metabolic Phenotypes, and the Function of Metabolic Tissues in Female Mice Fed A High-Fat Diet.
Amphiphysin 2 modulation rescues myotubular myopathy and prevents focal adhesion defects in mice.
Comparative proteomic analyses of human adipose extracellular matrices decellularized using alternative procedures.
Stromal collagen type VI associates with features of malignancy and predicts poor prognosis in salivary gland cancer.
Defining the hierarchical organisation of collagen VI microfibrils at nanometre to micrometre length scales.
Collagens VI and XII form complexes mediating osteoblast interactions during osteogenesis.
Acquired Localized Cutis Laxa due to Increased Elastin Turnover.
The long pentraxin PTX3 promotes fibrocyte differentiation.
Hypertensive rat lungs retain hallmarks of vascular disease upon decellularization but support the growth of mesenchymal stem cells.
Barbariga M, Vallone F, Mosca E, Bignami F, Magagnotti C, Fonteyne P, Chiappori F, Milanesi L, Rama P, Andolfo A, Ferrari G
Scientific reports 2019 Oct 3;9(1):14272
Scientific reports 2019 Oct 3;9(1):14272
Genetic Deletion of Syndecan-4 Alters Body Composition, Metabolic Phenotypes, and the Function of Metabolic Tissues in Female Mice Fed A High-Fat Diet.
De Luca M, Vecchie' D, Athmanathan B, Gopalkrishna S, Valcin JA, Swain TM, Sertie R, Wekesa K, Rowe GC, Bailey SM, Nagareddy PR
Nutrients 2019 Nov 18;11(11)
Nutrients 2019 Nov 18;11(11)
Amphiphysin 2 modulation rescues myotubular myopathy and prevents focal adhesion defects in mice.
Lionello VM, Nicot AS, Sartori M, Kretz C, Kessler P, Buono S, Djerroud S, Messaddeq N, Koebel P, Prokic I, Hérault Y, Romero NB, Laporte J, Cowling BS
Science translational medicine 2019 Mar 20;11(484)
Science translational medicine 2019 Mar 20;11(484)
Comparative proteomic analyses of human adipose extracellular matrices decellularized using alternative procedures.
Thomas-Porch C, Li J, Zanata F, Martin EC, Pashos N, Genemaras K, Poche JN, Totaro NP, Bratton MR, Gaupp D, Frazier T, Wu X, Ferreira LM, Tian W, Wang G, Bunnell BA, Flynn L, Hayes D, Gimble JM
Journal of biomedical materials research. Part A 2018 Sep;106(9):2481-2493
Journal of biomedical materials research. Part A 2018 Sep;106(9):2481-2493
Stromal collagen type VI associates with features of malignancy and predicts poor prognosis in salivary gland cancer.
Angenendt L, Mikesch JH, Görlich D, Busch A, Arnhold I, Rudack C, Hartmann W, Wardelmann E, Berdel WE, Stenner M, Schliemann C, Grünewald I
Cellular oncology (Dordrecht) 2018 Oct;41(5):517-525
Cellular oncology (Dordrecht) 2018 Oct;41(5):517-525
Defining the hierarchical organisation of collagen VI microfibrils at nanometre to micrometre length scales.
Godwin ARF, Starborg T, Sherratt MJ, Roseman AM, Baldock C
Acta biomaterialia 2017 Apr 1;52:21-32
Acta biomaterialia 2017 Apr 1;52:21-32
Collagens VI and XII form complexes mediating osteoblast interactions during osteogenesis.
Izu Y, Ezura Y, Koch M, Birk DE, Noda M
Cell and tissue research 2016 Jun;364(3):623-635
Cell and tissue research 2016 Jun;364(3):623-635
Acquired Localized Cutis Laxa due to Increased Elastin Turnover.
Nygaard RH, Maynard S, Schjerling P, Kjaer M, Qvortrup K, Bohr VA, Rasmussen LJ, Jemec GB, Heidenheim M
Case reports in dermatology 2016 Jan-Apr;8(1):42-51
Case reports in dermatology 2016 Jan-Apr;8(1):42-51
The long pentraxin PTX3 promotes fibrocyte differentiation.
Pilling D, Cox N, Vakil V, Verbeek JS, Gomer RH
PloS one 2015;10(3):e0119709
PloS one 2015;10(3):e0119709
Hypertensive rat lungs retain hallmarks of vascular disease upon decellularization but support the growth of mesenchymal stem cells.
Scarritt ME, Bonvillain RW, Burkett BJ, Wang G, Glotser EY, Zhang Q, Sammarco MC, Betancourt AM, Sullivan DE, Bunnell BA
Tissue engineering. Part A 2014 May;20(9-10):1426-43
Tissue engineering. Part A 2014 May;20(9-10):1426-43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Collagen VI alpha 1 Antibody [NB120-6588] - Showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Image using the Biotin format of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Collagen VI alpha 1 Antibody [NB120-6588] - Showed strong staining in FFPE sections of human placenta (Left) with red staining of stromal and extracellular spaces, and in testis (Right) with staining of extracellular spaces between seminiferous tubules). Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Collagen VI alpha 1 Antibody [NB120-6588] - Feline blood vessels section. The image was captured with an epifluorescent microscope. Image submitted by a verified customer review
