FNab10776

antibody from FineTest Biotech

Targeting: DNMT1 CXXC9, DNMT, MCMT

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

FNab10776

Provider

FineTest Biotech

Product name

DNMT1 antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes(TSGs) or other tumor-related genes in colorectal cancer(CRC) cells(PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells(ESCs)(PubMed:24623306). Associates at promoter regions of tumor suppressor genes(TSGs) leading to their gene silencing(PubMed:24623306). Promotes tumor growth(PubMed:24623306).

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

DNA(cytosine-5-)-methyltransferase
1


Epitope

IgG

Isotype

IgG

Vial size

100¦Ìg

Concentration

Immunogen affinity purified

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)

Handling

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20¡æ for 12 months(Avoid repeated freeze / thaw cycles.)