M00008

antibody from Boster Biological Technology

Targeting: APC DP2, DP2.5, DP3, PPP1R46

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00008

Provider

Boster Biological Technology

Product name

Anti-APC Monoclonal Antibody

Antibody type

Monoclonal

Description

Rabbit IgG Monoclonal antibody for P25054 detection. Tested positive for WB IHC IF/IF IP in Human Rat

Reactivity

Human, Rat

Host

Rabbit

Antibody clone number

FAD-1

Vial size

100ug

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of APC using anti-APC antibody (M00008) in human colon cancer samples.APC was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-APC Antibody (M00008) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image