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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- SM6010 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM6010, RRID:AB_1008511
- Product name
- anti VHL
- Antibody type
- Monoclonal
- Antigen
- Recombinant human His-VHL (1-154 aa) purified from E. coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 52A11
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis. HepG2 cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VHL antibody (1:500). Protein wasvisualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: SM6010 VHL antibody staining of Formalin-Fixed, Paraffin-Embedded Human Pancreas at 15 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
